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Nexus Kedokteran Translasional
Published by Universitas Sebelas Maret
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Core Subject : Health,
Medicine & Pharmacology
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Articles 18 Documents
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Search results for , issue "Vol 2, No 1 (2013): Nexus Kedokteran Translasional" : 18 Documents clear
Pengaruh Ekstrak Daun Teh (Camellia sinensis, Linn. var. Assamica) terhadap Mortalitas Cacing Ascaris suum, Goeze Afiyyuddin N, Ahmad; Utari, Cr. Siti; Wijayanti, Lilik
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Camellia sinensis, Linn. var. Assamica leaves contain tannin that have been known to have anthelmintic effect. This study aimed  to determine the effect of Camellia sinensis, Linn. var. Assamica leaves extract toward death time of Ascaris suum, Goeze. Methods: The study was a laboratory experimental research using the post-test only controlled group design. Subjects were Ascaris suum, Goeze. The sampling technique used was purposive random sampling. Subjects were divided into 7 groups, each group using 4 worms, replication performed 5 times. Saline solution was used in negative control group, pirantel pamoate 5 mg/ml was used in positive control group, while the treatment group used Camellia sinensis, Linn. var. Assamica leaves extract concentration of 50%, 60%, 70%, 80%, and 90%. Worms immersed in the test solution at 25 ml and incubated at 37°C. Observations were made every 1 hour and counted dead times of all worms. Data were analyzed with regression linier and probit analysis. Results: Observations of total deaths mean time Ascaris suum, Goeze negative control for 48 hours, the positive control 1 hours 10 minutes, the concentration of 50%, 60%, 70%, 80%, 90% for 10 hours 24 minutes, 9 hours 12 minutes, 7 hours 12 minutes, 5 hours 12 minutes and 4 hours. Linear regression test results show that the variation of the concentration affects long worm death. Probit analysis results obtained LC50 and LT50 Camellia sinensis, Linn. var. Assamica leaves extract is 67,183% and 3 hours 46 minutes. Conclusions: Based on this study, it can be concluded that of Camellia sinensis, Linn. var. Assamica leaves extract affects mortality of Ascaris suum, Goeze, the increasing of extract concentration is inversely proportional with the death time of worms. Keywords: Camellia sinensis, Linn. var. Assamica leaves extract, Ascaris suum, Goeze. 
Efek Diuresis Ekstrak Seledri (Apium graviolens L.) pada Tikus Putih Jantan (Rattus norvegicus) Evanti, Annisa Marsha; Doewes, Muchsin; Murti, Bhisma
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Celery (Apium graviolens L.) is known has some biochemical substances effect such as kalium nitrat, manitol, and apigenin/flavonoid which can result diuretic effect. This research aims at finding out the diuretic effect of celery extract (Apium graviolens L.) on the male white rats (Rattus norvegicus). Methods: The research is a laboratory experimental analytic study using the Randomized Controlled Trial design. The research subject is a number of 30 male white rats of Wistar Strain, 200 grams of body weight. They were divided into five treatment groups randomly. Group I was given 3 mL aquadest as negative control, group II was given 0,32 mg/3 mL hydrochlorotiazid as positive control, group III was given 1,2g/3 mL celery extract as dose I, group IV was given 2,4g/3 mL celery extract as dose II, and group V was given 4,8g/3 mL celery extract as dose III. Rats urine volume was measured every 4 hours for 16 hours. Results:The result of statistic calculation using ANOVA test with p = 0.000 (p < 0.001) shows that there are significant differences in total rats urine volume between group I, II, III, IV, and V in every observation time. The Post-hoc test shows significant differences between negative control and three dosages of celery extract (p < 0.05), and the similarities between positive control and three dosages of celery extract (p > 0.05). Conclusions: Through observing amount of urine volume and statistic calculation, it can be concluded that there is diuretic effect of celery extract on the male white rats. Celery extract at dose 2,4 g/3 mL has the highest diuretic effect but celery extract at 1,2 g/3 mL has the optimum diuretic effect. Keywords: Celery extract, diuretic effect, hydrochlorotiazid
PerbedaanPengaruh Nefroprotektor Ekstrak Kunyit, Ekstrak Temulawak, dan Kombinasinya Terhadap Kerusakan Histologis Sel Ginjal Mencit yang Diinduksi Parasetamol Sanubari, Aulia Agung; Muthmainah, .; Isdaryanto, .
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Turmeric and xanthorrhiza have been known to contain curcumin, and specially xanthorrhiza also contain xanthorrhizol that may exhibit significant protection of kidney cells from free radicals. This study has carried out to evaluate difference nefroprotector effect between turmeric, xanthorrhiza, and combination turmeric and xanthorrhiza on histological damage of kidney cells that induced paracetamol in mice. Methods: Present study was experimental laboratory research with post test only controlled group design.Samples were 30 male Swiss webster mice (2-3 months old) weighing+20 g, and they were divided equally into 5 groups, 6 mice each group. Sampling technique in this research was incidental sampling.KK (-) and KK (+) were given aquadest for 14 days. KP1 was given turmeric extract with the dose of 2,8 mg/20 g body weight of mice, KP2 was given xanthorrhiza extract with the dose of 14 mg/20 g body weight of mice, and KP3 was given combination of turmeric extract with dose 2,8 mg/20 g body weight of mice and xanthorrhiza extract with dose 14 mg/20 g body weight of mice for 14 days.Paracetamol was given to groups ofKK (+), KP1, KP2, and KP3on the 12th, 13th, and 14th day. On day-15th, micewere sacrificedandkidneys weretakento makepreparationsby paraffinblockmethodandHEstaining. Kidney histological features were assessed based on quantifying ofpyknosis, karyorrhexis, and karyolysis. Data were analyzed with the Kruskall-Wallis test (α=0.05) and continued with Mann-Whitney test (α=0.05). Results: Mean KK(-) : 10,33±1,40, KK(+) : 25,88±3,19, KP1 : 15±2,37, KP2 : 11,58±2,33, and KP3 : 13±1,92. Result of statistically data analysis showed that there was a significant difference of kidney damage score between all pairs of groups, except KK(-) - KP2 was not significant. Conclusion: There was a difference nefroprotector effect between turmeric, xanthorrhiza, and combination of them on histological damage of kidney cells that induced paracetamol, and xanthorrhiza extract at dose 14 mg/20 g body weight of mice had a better effect than turmeric extract or combination of them. Keywords: turmeric extract, xanthorrhiza extract, nefroprotector, kidney cells damage.
Pengaruh Pemberian Ekstrak Tempe Kedelai Terhadap Kerusakan Sel Ginjal Mencit (Mus musculus) Akibat Pemberian Parasetamol Ismanaf, Dadang; Widjokongko, S. B.; Sjarifah, Ipop
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background:The objectives of this research are to know the influence of soy bean tempeh extract to renal cell damaging of mice (Mus musculus) which is induced by Paracetamol and the increase of soy bean tempeh extract dose can  also increase protection effect  to renal cell damaging of mice (Mus musculus) which is induced by  Paracetamol.   Methods:This was laboratory experimental research with thepost test only controlled group design. Samples were 28 male mice, Swiss webstertype, 2-3 month sold age and ± 20 gr of each weight. Samples were divided into 4 groups of 7 mice each. Sampling technique used in this research was incidental sampling. Mice for control group (K) and the first treatment group (P1) will be given aquadest for 14 days in a row. The second treatment group (P2) and third treatment group (P3) will begiven soy bean tempeh extracts dose for 14 days in a row. Soy bean tempeh extracts dose in each group is 2,2 mg/20 gr weight of mice and 4,4 mg/20 gr weight of mice. Paracetamol  will be given to P1, P2, and P3, with dose 5mg/20 gr weight of mice in 0,1 ml aquadest on the day 12, 13, and 14. On day-15, mice were sacrificed and kidneys were taken to make preparations by paraffin block method and hematoxilin eosin (HE) staining. Kidney histological features were assessed based on quantifying of pyknosis, karyorrhexis, and karyolysis. Data were analyzed using the One-Way ANOVA test (α=0.05) and continued with Post Hoc Multiple Comparisons LSD test (α=0.05). Results:Result of One-Way ANOVA shows that there was a significant of degree between 4 groups with p=0,000 (p<0,05). Result of LSD method there was a significant of degree between K-P1, K-P2, K-P3, P1-P2, P1-P3, and P2-P3 groups with Beach p=0,000 (p<0,05). Conclusions:The feeding of soy bean tempeh extracts was able to decrease the renal cell damaging of mice and the increase of soy bean tempeh extracts dose     followed by the increase of protection effect to the renal cell damaging of mice which is induced by paracetamol. Keywords:soy bean tempeh extracts, paracetamol, renal cell damaging  
Pengaruh Pemberian Topikal Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) Terhadap Jumlah Sel Fibroblas Pada Penyembuhan Luka Sayat Pada Kulit Tikus Putih (Rattus norvegicus) strain wistar Aulia, Dentiko Wasis; Wasita, Brian; Pesik, Riza Novierta
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Binahong (Anredera cordifolia (Ten.) Steenis) is a common plant in Indonesia and contain many secondary metabolites such as flavonoids, tannins, saponins, terpenoids, and alkaloids. These secondary metabolite substances were expected to increase the number of fibroblast cells in the wound healing process. This study aims to demonstrate that topical administration of binahong leaf extract jelly on Wistar rats (Rattus norvegicus) skin cut will increase the number of fibroblasts in these wound healing process.   Methods: This is a laboratoric experimental study with randomize post test only control group design. The samples were 25 male Wistar rats aged 3 months and given a dorsal incision of 2 cm long and 0.5 cm deep. The rats were divided randomly into 5 groups (positive control, negative control, and 3 treatment group) consisting of 5 rats each. Positive control group were topically treated with patent medicine Bioplacenton®. Negative control group was not given any treatment. Treatment groups were topically treated with a binahong leaf extract jelly 2.5%, 5%, and 10% respectively. Bioplacenton® and binahong leaf extract jelly is given 2 times a day on its wound for 5 days. On the fifth day, all rat’s wound were scored using Bates-Jensen Wound Assassment Tool and then sacrificed to make histophatology slides on the part of the injured skin. The slides were stained with Van Giesson staining and the fibroblast in the granulation tissue was calculated. The data obtained was analyzed with Oneway ANOVA (p <0.05).   Result: The macroscopic wound scoring and average number of fibroblast cells in each group showed no significant difference (p>0.05). However, in the 2nd treatment groups (binahong leaf extract jelly 5%) showed the highest number of fibroblasts (153.72 (±SD 23.39)) than the other groups. Conclusion: Topical administration of binahong leaf extract (Anredera cordifolia (Ten.) Steenis) did not affect the macroscopic wound scoring but it increase the number of fibroblast cells in healing skin cuts on Wistar rats (Rattus norvegicus) even thought it did not reach the significant level. Keywords: wound healing, fibroblast, binahong (Anredera cordifolia (Ten.) Steenis)
Pengaruh Ekstrak Etanol Belimbing Wuluh (Averrhoa bilimbi Linn.) dengan Variasi Dosis terhadap Kadar Testosteron Bebas Tikus Putih (Rattus norvegicus) Jannah, Nur Zahratul; Doewes, Muchsin; Kisrini, .
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Belimbing wuluh (Averrhoa bilimbi Linn.) contains apigenin and luteolin as natural flavonoids, and antioxidants that may increase testosterone levels. Apigenin and luteolin increase testosterone levels by enhancing StAR (Steroidogenic Acute Regulatory) activity. Antioxidant protects cell from oxidative stress caused by ROS (Reactive Oxygen Species). This study aimed to evaluate the effect of variation dosage of Belimbing Wuluh (Averrhoa bilimbi Linn.) ethanolic extract on free testosterone levels in rats (Rattus norvegicus) Methods: This  was a pure experimental study with randomized post test only controlled group design. Samples were 24 male Wistar strain rats, 8-10 weeks aged and weighted ± 200 g on average. Samples were randomly divided into 4 groups, each group consists of six rats. Group K were given aquadest for 14 days. Group P1, P2, P3 were given ethanolic extract of belimbing wuluh orally at dose 57 mg/200 g body weight of rats, 114 mg/200 g body weight of rats, and 228 mg/200 g body weight of rats for 14 days. At 15th, blood were drawn from retroorbitalis plexus using microtubuler tube and free testosterone level were measured. Free testosterone levels of rats were measured by using ELISA (Enzyme-linked Immunosorbent Assay) method. Data were analyzed using one-way ANOVA test (α = 0.05). Results: Mean free testosterone levels in group K was 2.40 ± 2.02 ng/ml; in group P1 was 1.76 ± 1.01 ng/ml, in group P2 was 1.58 ± 0.53 ng/ml and in group P3 was 1.68 ± 0.64 ng/ml. Results for one-way ANOVA test showed no mean different (p=0.642). Conclusions: There were no effect between variation dosage of belimbing wuluh (Averrhoa bilimbi Linn.) ethanolic extract on free testosterone level in rats (Rattus norvegicus). Keywords: Averrhoa bilimbi Linn. extract, flavonoid, free testosterone level
Pengaruh Pemberian Sari Buah Stroberi Terhadap Kerusakan Histologis Sel Ginjal Mencit Akibat Paparan Parasetamol Komalasari, Pratita; Muthmainah, .; Murti, Bhisma
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Strawberry essence is known to contain vitamin C, anthocyanins and elagic acid. It has potent antioxidant properties and anti-inflammatory. This study aims to examine the effects of strawberry essence in preventing histologic damage renal cell of mice (Mus musculus). Methods: The study used Randomized Controlled Trial (RCT) design. The samples of 28 male mice (Mus musculus) Swiss Webster strain, aged 2-3 months, body weight ± 20 grams were divided into 4 groups: KK (aquadest), KP I (aquadest + paracetamol), KP II (Paracetamol+Strawberry essence dose 416 mg/20 g BB), KP III (Paracetamol+Strawberry essence dose 832 mg/20 g BB). The treatment was given for 14 days, except paracetamol was given only on day 12 th, 13 th, 14 th. On day 15th mice were sacrificed by neck dislocation. Then right and left renal of mice were taken. From each renal was made two histological preparations. Renal preparations were made ​​by the method of paraffin blocks with Haematoxylin Eosin staining (HE). Observations of renal used a light microscope magnification of 1000 times. The number of damaged cells from each of 50 cells in the renal proximal tubule were counted. Data were analyzed by One-Way Analysis of Variant Test (ANOVA) and Post Hoc test (α=0,05). Results: Mean of KK = 7.61 ± 1.786, KP I = 37.07 ± 2.905, KP II = 29.64 ± 3.540, KP III = 22.39 ± 3.403. There were significant differences of the mean number of damage cells in all pairs of groups (KK-KP I, KK-KP II, KK-KP III, KP I-KP II, KP I KP III, and KP II-KP III). Conclusion: The Strawberry essence (Fragaria x annanassa) could prevent histological damage renal cells of mice exposed to paracetamol, but it could not give the same effect as the KK (p < 0.001). Keywords: Strawberry Essence, paracetamol, histologic damage renal cell
Efek Nefroprotektif Ekstrak Etanol Buah Jambu Biji Merah (Psidium guajava Linn) terhadap Kerusakan Histologis Ginjal Mencit yang Diinduksi Asetaminofen Priscilla, Prisca; Suyatmi, .; Sari, Yulia
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Red guava fruit (Psidium guajava Linn) contains various of antioxidants such as vitamins A, C, E, minerals Fe, Zn, Se, flavonoids, and lycopene, which they were thought to have nephroprotective effect. The objective of this research was to evaluate the preventive effect of red guava fruit ethanolic extract on tubular renal damage induced by acetaminophen on mice. Methods: Samples were twenty eight male mice, Swiss webster strain, 2-3 months old age and + 20 gram of each weight. Sample divided into 4 groups, each group consists of seven mice. The negative (KK(-)) and positive control group (KK(+)) mice were given aquadest for 14 days. The other group of mice were given red guava fruit ethanolic extract with the dose of 35 mg/20 g body weight of mice (KP1) and the last group of mice were given red guava fruit ethanolic extract with the dose of 70 mg/20 g body weight (KP2) for 14 days. Acetaminophen was given to groups of KK(+), KP1, and KP2. The 15th day, mice were sacrificed and histological preparation were made to evaluate histological damage on rens. Renal histological features were assessed by counting the number of tubular epithelial undergoing pyknosis, karyorrhexis, and karyolysis. Data were analyzed using the One-Way ANOVA test (α = 0.05) and then were continued with Post Hoc Multiple Comparisons (LSD) test (α = 0.05). Results: The mean renal histological damage score was significantly higher on group KK(+)= 40.50+1.991, while the group of KK(-)= 8.93+1.720 as the less. The group of KP2 had fewer numbers of renal histological damage (17.96+1.621) compared to the KP1 group (25.36+1.929). The results of One-Way ANOVA and LSD test showed that there was significant difference between the four groups (p = 0.000). Conclusions: The ethanolic extract of red guava fruit showed nephroprotective effect to the renal histological damage of mice which was induced by acetaminophen. Red guava fruit ethanolic extract with the dose of 70 mg/20 g body weight of mice showed a higher nephroprotective effect than the dose of 35 mg/20 g body weight of mice. Keywords: ethanolic extract, red guava, acetaminophen, renal histological, mice.
Efek Analgesik Ekstrak Daun Kemangi (Ocimum sanctum L.) terhadap Tikus Putih (Rattus norvegicus) Agrensa, Riza Setya; Doewes, Muchsin; Samigun, .
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Pain is a symptom of a disease that many felt by the people, the prevalence of pain in adults reach to 40% per day, while 89% felt the pain episode at least once a month. To overcome the pain that arises, people often use drugs is to reduce pain or analgesic. This research aims to find out whether there is an analgesic effect of kemangi extract (Ocimum sanctum L.) against the white mice (Rattus norvegicus). Methods: This research uses experimental methods to the post test only control group design. The research at the University of Setia Budi Surakarta performed. The subject of this research is 25 tail of a white mice (Rattus norvegicus), 3 months old with average weight 200 grams. First, subject adapted for 1 week, then not given to eat and drink for 18 hours before the treatment. The subject is divided into 5 groups. The negative control group was given aquades, while the positive control group was given aspirin. The third treatment group each got kemangi extract dosage of 225 mg/200 g body weight, 450 mg/200 g body weight, and 675 mg/200 g body weight. Pain induction method used is a medium hot plate, and then calculated the frequency jumps. The data obtained and analyzed by One-Way ANOVA and LSD. Results: The results of One-Way ANOVA test showed a significant difference between the five treatment groups. LSD test results showed a significant difference between the negative with the positive control group. Positive control group treated with the first and second doses showed a significant difference, but with the third dose treatment group showed no significant difference. This shows that only the third dose group has the analgesic effects. Conclusions: Of the three groups treated with kemangi extracts, only the third dose treatment groups shows analgesic effect on white mice. Keywords: kemangi extract, analgesic effects
Pengaruh Ekstrak Etanol Belimbing Wuluh (Averrhoa bilimbi Linn.) dengan Variasi Dosis terhadap Kadar Testosteron Bebas Tikus Putih (Rattus norvegicus) Nur Zahratul Jannah; Muchsin Doewes; . Kisrini
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Belimbing wuluh (Averrhoa bilimbi Linn.) contains apigenin and luteolin as natural flavonoids, and antioxidants that may increase testosterone levels. Apigenin and luteolin increase testosterone levels by enhancing StAR (Steroidogenic Acute Regulatory) activity. Antioxidant protects cell from oxidative stress caused by ROS (Reactive Oxygen Species). This study aimed to evaluate the effect of variation dosage of Belimbing Wuluh (Averrhoa bilimbi Linn.) ethanolic extract on free testosterone levels in rats (Rattus norvegicus) Methods: This was a pure experimental study with randomized post test only controlled group design. Samples were 24 male Wistar strain rats, 8-10 weeks aged and weighted 200 g on average. Samples were randomly divided into 4 groups, each group consists of six rats. Group K were given aquadest for 14 days. Group P1, P2, P3 were given ethanolic extract of belimbing wuluh orally at dose 57 mg/200 g body weight of rats, 114 mg/200 g body weight of rats, and 228 mg/200 g body weight of rats for 14 days. At 15th, blood were drawn from retroorbitalis plexus using microtubuler tube and free testosterone level were measured. Free testosterone levels of rats were measured by using ELISA (Enzyme-linked Immunosorbent Assay) method. Data were analyzed using one-way ANOVA test (? = 0.05). Results: Mean free testosterone levels in group K was 2.40 2.02 ng/ml; in group P1 was 1.76 1.01 ng/ml, in group P2 was 1.58 0.53 ng/ml and in group P3 was 1.68 0.64 ng/ml. Results for one-way ANOVA test showed no mean different (p=0.642). Conclusions: There were no effect between variation dosage of belimbing wuluh (Averrhoa bilimbi Linn.) ethanolic extract on free testosterone level in rats (Rattus norvegicus). Keywords: Averrhoa bilimbi Linn. extract, flavonoid, free testosterone level

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